Propagation of Rosa “souvenir de la malmaison” by tissue culture techniques

Abstract

    In this study, stem segments of rose were used as explants. The results showed that nodal stems were surface disinfected by immersing 70% etanol in 10 minutes, then treating in 5% javel solution, the rate of living-disinfected explants reached 79.0%. The medium for in vitro generating shoot from domacy buds of stem was MS, 30 g/L sucrose, 7 g/L agar and added 1.0 mg/L BAP. The same medium being added 2.0 mg/L BAP was suitable for shoot multiplication, in which the average shoot number per explant, the average length of shoot were 6.0 and 4.70 (cm), respectively. The ½ MS, 30 g/L sucrose, 7 g/L agar and supplemented 0.75 mg/L NAA was effective for in vitro root formation, the rooting percent of microshoots reached 75.0%. In vitro plantlets were acclimatized by growing in TS1 mixture substrate which had the highest survival rate (68.4%) after 2 weeks hardening.