Molecular characteristics of ITS-rDNA region of Mahonia bealei (Fortune) Carrière in Vietnam

DOI: 10.18173/2354-1059.2021-0013

Abstract

In this research, we study on molecular characteristics of ITS-rDNA region of Mahonia bealei. The gotten result showed that, the ITS with 700 bp in length contained 24.1% Timin, 28.4% Guamin, 23.4% Adenin and 24.1% Cistezin. The average genetic difference between Mahonia genus and Berberis genus is 2,3% while with other genera is from 17% to 22%. The gotten result also demonstrated this ITS region can be useful and suitable for identification and study phylogenetic of Berberidaceae. Specific primer to amplify the ITS genome in the length of 300 bp of Mahonia bealei species aslo have been developed and synthesized. This ITS-300 genome sequence of Mahonia bealei was registered on the Gene Bank with accession number as MT 008067.