Hairy root induction from Impatiens balsamina L. using fourteen Agrobacterium rhizogenes strains

Abstract

Impatiens balsamina L. is a plant commonly grown in Vietnam. It has long been used as traditional medicine. All of the secondary metabolites produced by Impatiens balsamina L. root possessed biological activities. The main advantage of using hairy root cultures is their ability of growing fast in defined basal media without supplementation of phytohormones. The aim of this research is to invest some factors that could affect on the hairy root production such as A. rhizogenes strain, type of tissue, OD concentration, immersion time and co-cultivation time. The results showed that 3 strain A. rhizogenes C02, C18 and C26 isolated from nature in Vietnam could induce the best hairy root formation at the 0.5–1.0 OD concentration bacteria with 5’ of immersion time and 72 hours of co-cultivating. Among them, C02 is the strain that could offer the best result in B5 medium.