DETERMINATION OF CARBAMATE RESIDUES IN VEGATABLES, BULBS AND WATER

Abstract

A method for the determination of residues of 10 carbamates including Aldicard Sulfoxide, Aldicarb sulfone, Oxamyl, Methomyl, 3-Hydroxycarbofuran, Aldicarb; Propoxur, Carbofuran, Carbaryl and Methiocarb in vegetables, bulbs and water was established based on High Performance Liquid Chromatography with fluorescence detection after derived post column. Solid samples were grinded in MeOH, clarify. Extraction liquid or water samples were cleaned by liquid-liquid extraction or SPE C₁₈. Carbamates were extracted in HPLC C₁₈ with mobile phase gradient MeOH/H₂O and derived with O – phthalaldehyde (OPA) and 2 – mercaptoethanol. A fluorescent compound 1 – hydroxytylthio – 2 – metylisoindol was produced and detected by fluorescence detection with λ_ex 340 nm and λ_em 445 nm. Limit of detection and limit of quantitation for the carbamates were 0.51 - 5.0 ppb and 1.69 – 9.09 ppb, respectively.  The recoveries of the carbamates were from 73 to 95%. The concentrations of the carbamate in vegetables, bulbs and water at some investigated sites were low.