Chemical composition analysis and antibacterial-antiinflammatoryactivity tests of tamanu seed oil extracted by supercritcial fluid technology

Abstract

The natural product of tamanu oil is valuable in medicine and cosmetic. In this study, the tamanu oil was extracted by supercritical CO2 (sCO2) and Soxhlet (using hexane solvent) methods. The fatty acids and coumarins compound of the obtained tamanu oil were analyzed by gas chromatography–mass spectrometry (GC-MS). The antibacterial and anti-inflammatory activities were also investigated by the diffusion method and performed on the white mice ears (Swiss albino). Content of fatty acid in tamanu oil extracted using sCO₂ (72.62 %) was higher than that using Soxhlet method. The compound of coumaron (2H-1-bezopyran-2-one, C₉H₆O₂) was identified belonging the simple coumarin group and its content in the tamanu oil extracted using sCO₂ was the highest (169.69 µg/g), 1.79 and 2.04 times as much as that using Soxhlet method and the commercial oil, respectively. Besides, the results of investigation of the antibacterial activity suggested that the tamanu oil extracted using sCO₂ was able to against some of strains namely: Staphylococcus aureus (S. aureus) ATCC 25923, S. aureus ATCC 29213, methycilin-resistant S. aureus (MRSA) ATCC 43300, and Pseudomonas aeruginosa (P. aeruginosa) ATCC 277853.

 In term of the anti-inflammatory activity, the tamanu oil extracted using sCO₂ was anti-inflammatory effectively with reducing flammatory: 21.41 – 25.39 % as for the thickness of mice ears and 33.88 – 39.40 % as for the weight of mice ears.