Studying on growth of strawberry (Fragaria ananassa l.) cell suspension cultures for anthocyanin production

Abstract

Cell suspension cultures were initated from calli derived from in vitro strawberry leaves on MS medium (Murashige and Skoog, 1962) supplemented with 30 g/l sucrose, 1.0 mg/l 2,4-D and 0.3 mg/l kinetin. There were many factors affected on cell suspension cultures growth (it was found that …). Cell suspension cultures grew better on MS medium with 30 g/l sucrose. 1 g (fresh weight) of cells in 20 ml of medium was the best initial inoculum cell density for cell suspension cultures to grow. A shaking speed of 100 rpm on rotary shaker was suitable for the cells. The growth of cell suspension in dark was better than that under light condition. Anthocyanin in the cells was determined by pH differential method.