PHÁT TRIỂN HỆ NHŨ TƯƠNG NANO CHỨA TINH DẦU TRẦU KHÔNG VÀ ĐÁNH GIÁ HOẠT TÍNH KHÁNG NẤM Malassezia furfur

Tóm tắt

This study evaluated the antifungal activity against Malassezia furfur of Piper betle L. essential oil by agar
diffusion assay. The inhibition zone diameter of P. betle essential oil was 21.7± 1.31mm (50 mg/mL), 14.1 ±
0.63 mm (25 mg/mL) and 10.2 ± 0.35 mm (12.5 mg/mL). However, essential oils have low solubility in water
and are unstable by environmental factors. This study also developed the self-nanoemulsifying drug delivery
system (SNEDDS) containing P. betle essential oil to enhance its stability and solubility. The composition of
the nanoemulsion included P. betle essential oil (1 % v/v) and capric triglyceride (1 % v/v) as the dispersion
phase, distilled water (92 % v/v) as the continuous phase, non-ionic surfactant Tween 80 (4.8 % v/v) along
with 1,3-propanediol (1.2 % v/v) as the emulsifying agents. Dynamic light scattering (DLS) measurements
showed that the P. betle nanoemulsion had a mean droplet size that meets the size requirements of the
SNEDDS and remained relatively stable for 42 days of storage. In comparison, the minimum inhibitory
concentration (MIC) of P. betle essential oil in the nanoemulsion against M. furfur was 0.625 mg/mL, twice
as high as the MIC of P. betle essential oil dissolved in DMSO. In conclusion, the nanoemulsion containing
P. betle essential oil has potential applications in antifungal activity against M. furfur. However, the stability
in antifungal ability of nanoemulsion needs to be determined more clearly in future studies.