HOẠT TÍNH KHÁNG OXY HÓA VÀ KHÁNG VIÊM IN VITRO CỦA CÁC CAO CHIẾT LÁ CÂY CÒ SEN (Miliusa velutina)

Tóm tắt

Miliusa velutina leaf contains a promising source of bioactive compounds since it has been
traditionally used for the treatment of various diseases. The present study aimed at evaluating the effect
of different solvents on phytochemical constituents, antioxidant and in vitro anti-inflammatory activities
of Miliusa velutina leaves extracts. Aqueous solvent was identified as the most effective one for the
extraction, resulting as the highest content of polyphenol (13.85±0.13 mg GAE/g extract) and flavonoid
(267.34±4.93 mg QE/g extract). In addition, the study was conducted to evaluate the antioxidant and
anti-inflammatory activities of Miliusa velutina leaves extracts. The antioxidant activity was determined
using nitric oxide radical inhibition assay. In vitro anti-inflammatory activity was evaluated using
albumin denaturation method. The results showed that the aqueous extract exhibited high capacity of
antioxidant (EC50 value of 157.08±2.90 μg/mL) and in vitro anti-inflammatory activity (i.e., albumin
denaturation: IC50=21.13±0.95 μg/mL). The antioxidant activity of the aqueous extract was found to be
4.63-fold higher than vitamin C, and the anti-inflammatory activity of Miliusa velutina extracts was
comparable to diclofenac and prednisolon. Based on the obtained results, it can be concluded that
Miliusa velutina leaves extracts displayed anti-inflammatory and antioxidant activity.
Keywords: Anti-inflammatory, antioxidant, diclofenac, Miliusa velutina, nitric oxide, prednisolone