TỔNG HỢP XANH NANO BẠC SỬ DỤNG DỊCH CHIẾT LÁ CHÈ VÀ ĐÁNH GIÁ HOẠT TÍNH KHÁNG KHUẨ

Tóm tắt

In this study, the silver nanoparticles (AgNPs) were successfully synthesized via a simple, nontoxic and
ecofriendly method by using tea leaf extract. The formation of AgNPs was identified by changing the
color of the reaction medium from light yellow to dark brown and the surface plasma resonance band.
The optimal conditions for the synthesis of silver nanoparticles were determined by changing various
parameters including pH, amount of extract, concentration of AgNO3 solution and time. Furthermore,
the biosynthesized AgNPs were characterized by Fourier transform infrared spectroscopy (FT-IR), Xray diffraction (XRD) and scanning electron microscopy (SEM). The AgNPs were nearly spherical in
shape with the crystalline size of about 10 nm. The active compounds in the extract containing
functional groups such as hydroxyl, amine, ether acted as a reducing agent to convert silver ions into
AgNPs and capping agent on the surface of AgNPs. Silver nanoparticles exhibited significant
antibacterial activity against P. aeruginosa and S. aureus with the inhibition zones ranging between
19-26 mm and 22-29 mm, respectively.