Research on the inhibitive ability Aspergillus niger causing collar rot disease in groundnut of some nano preparations

Abstract

Collar rot is a devastating disease caused by the soil-borne pathogen Aspergillus niger, which significantly

impacts groundnut production worldwide. The long-term persistence of this fungus in the soil can diminish the

effectiveness of synthetic fungicides. In this study, a compound made of Chitosan oligosaccharide 1000 ppm

(COS 1000 ppm) combined with Silver nanoparticles 50 ppm (AgNPs 50 ppm) and Copper ions 500 ppm (Cu2+

500 ppm) was tested for its antifungal activity against A. niger. The results showed that concentrations of the

AgNPs, Cu2+/COS nanoparticles compound ranging from 1 to 5% effectively inhibited the growth of A. niger in invitro conditions, achieving complete inhibition (100%). Additionally, in net-house conditions, applying the AgNPs,

Cu2+/COS nanoparticles at a concentration of 1% either one time (one day before inoculation) or two times (one

day before and one day after inoculation) reduced the disease rate from 100% to between 0% and 4.44%

compared to the control group. Therefore, AgNPs, Cu2+/COS nanoparticles appear to be a promising high-tech,

safe, and effective solution for preventing collar rot disease in groundnut caused by the fungus A. niger