Research on the effect of lignocellulose hydrolysis methods from pinus wood chips to recover glucose

Abstract

Glucose production from lignocellulosic biomass of pine wood chips is a key factor contributing to reducing the total cost in the process of converting biomass into ethanol and the enzymatic hydrolysis of cellulose brings more benefits than chemical treatment methods thanks to the flexible activity of enzymes, no environmental pollution, high efficiency and the creation of the least by-products. Steam enzymatic hydrolysis at high solid concentrations must overcome the resistance in the hydrolysis process, such as high reaction rates and high glucose concentrations. An optimal control strategy for the hierarchical hydrolysis process - batch has been studied to solve the above problems, conducting the incremental addition of substrate at the rate of 25 %/time (corresponding to being fed - batch 4 times) at times such as 0 h, 6 h, 12 h and 18 h. The cellulose conversion process is maximized and the maximum glucose concentration is accumulated during the incremental substrate time (fed - batch), reaching 190.1 g/L with a recovery efficiency of 70.7 %, the largest hydrolysis rate of 3.55 (g/L × h). After 96 hours of hydrolysis, the hydrolysis efficiency increased by 93.7 %, and the glucose concentration reached 252 g/L.