Studies on Agrobacterium tumefaciens - Mediated Transfer of RNAi to Dendrobium Sonia for Resistance to CyMV

Abstract

     In order to produce a Dendrobium lines resistant to CyMV (Cymbidium mosaic virus), protocorm like bodies (PLBs) of Dendrobium Sonia were infected with Agrobacterium tumefaciens C58 carrying plant transgenic vector pK7GWIWG2 (II)/CP, containing RNAi structure of coat protein gene (CP) alone or in combination with ORF2-4 gene of CyMV. The transformed PLBs were disinfected with cefotaxime 300 mg/l and screened for kanamycin 500 mg/l. Viable PLB-derived lines were obtained after the screening. The presence of the target gene in these lines was confirmed by PCR.