STUDY ON CHARACTERISTICS OF BEAUVERIA STRAINS ISOLATED FROM THE BACH CUONG TAM (BOMBYX MORI) IN TAM GIANG COMMUNE, YEN PHONG DISTRICT, BAC NINH PROVINCE

Abstract

Bach Cuong Tam (Bombyx botryticatus) is a traditional medicine dispensed from the silkworm (Bombyx mori L) after infection fungus Beauveria bassiana. From 05 samples of Bach Cuong Tam collected at Vong Nguyet Village, Tam Giang Commune, Yen Phong District, Bac Ninh Province, 11 strains of fungi have been isolated, of which 02 strains belong to the genus Beauveria. A Study on biological characteristics resulted in two strains with good growth ability at 20-30°C and pH from 5 to 7, assimilation of carbon sources including D-fructose, saccharose, lactose, mannitol, maltose, and D-glucose, and capable of producing several extracellular enzymes such as amylase, chitinase, and protease, the classification based on the 5.8S gene sequence resulted in two strains having 100% similarity with Beauveria bassiana and Beauveria amorpha strains. Two fungus strains were cultured on five-week-old mulberry silkworm (Bombyx mori L) and showed good growth after 7 days.