Study on making tyrosinase biosensor from Aspergillus oryzae TP01 and its application

  • Trịnh Thị Thu Hằng
  • Nguyễn Thị Xuân Sâm
  • Đỗ Thị Thu Huyền
  • Đặng Thị Thu

Abstract

Tyrosinase (EC 1.14.18.1) is a Polyphenol oxidase.  It is a bifunctional enzyme catalyzing the monohydroxylation of monophenols and the oxidation of diphenols. Tyrosinase is used to medicine, food industry and environmental protection, especially used to create the biosensor. Tyrosinase has been purified by Mono Q HR 5/5 column in FPLC system with the result of  18.96 folds as the rude enzym, its specific activity is 204.75 U/mgPr and its yield is 31.63%. Determining the optimum condition to immobilised the enzym on biosensor: the amout of  tyrosinase is 50U, duration of 30 minutes, BSA of 1.3mg in saturated glutaraldehyt, reutilization of 12 times. After 50 days the biosensor is preserved in the tinned bag  at 4o C, the output signal remains 79.51%. The  optimum  condition  of  the  biosensor  is  the  temperature  of  30o C, pH  6.6,  subtrate density  of  10-7 - 4  ×  10-4  M.  Biosensor  has  been  detected monophenol  compound from  the density of 10-7 M–3 × 10-4 M and diphenol compound from 10-6  M - 4 × 10-4 M. Application  of  tyrosinase  biosensor  has  been  detected  atrazin,  diuron,  2.4D  and  phenol compounds in waste water from the petrolium, chemical, tobacco, dying and soaping factories.
điểm /   đánh giá
Published
2014-10-29
Section
Articles