Isolation and selection of antagonistic bacteria to Ralstonia solanacearum caused bacterial wilt disease on groundnut and sesame

Abstract

Chemical  pesticides are widely  used  to control diseases. However, the use of  these pesticides can be expensive. They may also play a dominant role in  environmental pollution, such as groundwater contamination. Thus, using  biological control agents, especially biopesticieds, to fight such disease is gaining  increasing  momentum  in  the  agricultural biotechnology  research. This paper  bring out the  results of  isolation  and  selection  the antagonistic bacteria to make micro-product (biopesticieds) for controlling bacterial wilt disease on groudnut  and sesame. 10 isolates  of  antagonistic bacteria to Ralstonia solanacearum have selected,which are not harmful to plant and mice. Among them CX₅ belonging to  Bacillus  and PX₁ is Pseudomonas. The both CX₅ and PX₁ can control bacterial wilt disease on groundnut, sesame and Plant-Growth-Promoting Rhizobacteria  (PGPR). So these isolates are selected  to control bacterial wilt disease on groudnut and sesame. When using CX₅ strain reduced 66.64% of died sesame, 74.41%  groudnud; PX₁ reduced 71.31%  of  died  sesame  plant  and  61.3%  on groundnut, respectively.