CHARACTERISATION OF BACTERIAL DIVERSITY IN LAKE SEDIMENTS AT THE HERBICIDEIDIOXlN CONTAMINATED AREA OF DANANG FORMER MILITARY BASE BY PCR-DGGE TECHNIQUE

Abstract

PCR-DGGE technique was used for characterisation of bacterial community structure insediments of three lakes A, Band C of the herbicide/dioxin contaminated area in Danang former military base. There were differences on bacterial diversity level between sediment samples andtwo sampling time. In general, bacterial diversity level in sediments of lake A was higher incomparison with sediments of two other lakes Band C. Several strong and sharp bands wereappeared in one or some samples. The genetic coefficiencies of bacterial community insediments are ranged from 20% to 87.5%. The sequence analysis of several representative DNA bands in lake sediments showed high similarity to some uncultured clones, including Alphaproteobacteria, Chlorobi, Chloroflexi, Sulfuricurvum, Fusobacteria and Nitrospiraceae. Moreover, some band excised from DGGE gel was also showed a high levels to other unclassified bacterial clones.