Xác định đa dạng vi khuẩn trong bùn hồ khu vực nhiễm chất diệt cỏ chứa Dioxin tại sân bay Đà Nẵng bằng kĩ thuật PCR-DGGE

Tóm tắt

PCR-DGGE technique was used for characterisation of bacterial community structure insediments of three lakes A, Band C of the herbicide/dioxin contaminated area in Danang former military base. There were differences on bacterial diversity level between sediment samples andtwo sampling time. In general, bacterial diversity level in sediments of lake A was higher incomparison with sediments of two other lakes Band C. Several strong and sharp bands wereappeared in one or some samples. The genetic coefficiencies of bacterial community insediments are ranged from 20% to 87.5%. The sequence analysis of several representative DNA bands in lake sediments showed high similarity to some uncultured clones, including Alphaproteobacteria, Chlorobi, Chloroflexi, Sulfuricurvum, Fusobacteria and Nitrospiraceae. Moreover, some band excised from DGGE gel was also showed a high levels to other unclassified bacterial clones.