PRIMARY INVESTIGATION OF LISTERIA MONOCYTOGENES INFECTION IN FOODS IN HANOI MARKET USING POLYMERASE CHAIN REACTION
Abstract
Using PCR techniques developed with LM-F76. LM-R543 primers, the investigation of the
infection of L. monocylogenes was done with 165 food samples including 116 dairy- and 49
meat-based foods. The PCR analysis showed no contamination with L monocytogenes was
detected in dairy samples, in contrary. 11.^49 meat samples was positive with L. monocytogenes.
This result was \erified b\' identification of the strains isolated from positive sample on Oxford
agar with 16S rDNA technique. The result confirmed that all strains isolated from PCR positive
samples were identified as L. monocytogenes. This primary analysis gave a first warning to the
contamination risk of L. monocytogenes in food in Hanoi market. It demands a widely
investigation for the dead bacterium infection risk in the marketed foods.