Sequence analysis of partial nucleoprotein (N) gene of Rabies virus strain in Ha Tien, Kien Giang

Abstract

This study was performed with the aim of characterization of the rabies virus (genus: Lyssavirus
of the family Rhabdoviridae under the order Mononegavirales) by sequencing partial nucleoprotein
(N) gene of the rabies virus and phylogenetic analysis to know genotype and genealogy of rabies
virus 1230.2019 in the infected dogs in Ha Tien, Kien Giang province, Viet Nam. A brain sample
was aseptically collected from a dead dog to detect rabies virus. This sample was found to be
positive through RT-PCR assay, with high content genetic products through sequencing.
Arrangement of the nucleotide and amino acid sequences of the rabies strain 1230.2019 with
reference rabies strains showed the similarity of 95.20–100% and 92.00–100%, respectively.
Rabies virus strain 1230.2019 of infected dog in Ha Tien (Viet Nam) has the same genetic
relationship with eight reference rabies virus in the study. Strain 1230.2019 has genetic
distance analysis results (0-0.16%) very close to five reference rabies vaccine strains and three
strains of rabies virus in infected dogs in GenBank. Genealogy based on nucleoprotein (N)
gene sequence, strain 1230.2019 has almost absolute homogeneity and high similarity with
French vaccine strain 93127FRA (RABISIN vaccine against rabies is widely used in Viet Nam)