Survey on circulation of Japanese encephalitis virus in pigs in Gia Lam district, Ha Noi City

Abstract

RT-PCR (Reverse transcription polymerase chain reaction) method was applied to detect the presence of Japanese encephalitis virus (JEV) in the pig serum samples collecting from Gia Lam district and to investigate the role of pig in the natural infection cycle. The studied result showed that 6.25% (5/80) of the serum samples was positive with JEV. The length of prM gene of the studied JEV strains was 563 nucleotides and the similarity rate of them was from 99.29% to 99.82%; and the similarity rate on amino acid of them ranged from 98.27% to 100.0%. The studied result on the phylogenetic tree showed that five isolated JEV strains were the same origin with the previous isolation strain from Japan, Korea and China, all of them belonged to genotype 1. The results of this study contributed to show the infected situation of JEV in pig in Gia Lam district and to give the method in controlling JEV disease in pig.