Comparing the quality of the aluminium hydroxide and oil emulsion adjuvant, inactivated multivalent vaccines against pneumoniae in swine

Abstract

In this research, the master seed bacteria strains, including three strains of A. pleuropneumoniae, two strains of P.multocida and one strain of S. suis were approved by Department of Animal Health to produce the aluminum hydroxide adjuvant, inactivated multivalent vaccine, they were also used to develop an inactivated multivalent vaccine with oil emulsion adjuvant to prevent pneumoniae in swine.
Both vaccines (with aluminium hydroxide and oil emulsion adjuvant) were found to be good quality with 100% sterility and 100% safety, which was proved through the experimental tests on mice and pigs. Both vaccines passed the potency test with a protection rate of 80% on mice, using challenge method.
Using ELISA technique, it was detected that both vaccines stimulated the pigs in producing antibodies to A. pleuropneumoniae serotypes 2 and 5 with high titers after 21 days of vaccination in pigs from four weeks of age. The antibody titer of oil emulsion adjuvant vaccine was higher than that of the aluminium hydroxide adjuvant vaccine.