Research on adding skim milk powder and vitamin C in liquid preservation extender for Phu Quoc dog semen

Abstract

The study was conducted to access the suitable levels of skim milk powder and vitamin C adding in liquid preservation extender for Phu Quoc dog semen. This liquid preservation extender name was TCF. The test levels of skim milk powder and vitamin C supplementing in TCF were 0; 15; 30, 60 mg/ml and 0; 10; 20; 30 mg/ml, respectively. The preservation temparature for semen was 5oC, the rate of semen dilution was 1:5. The sperm motility and abnormality were evaluated on the 1st, 2nd and 3rd day after preservation. The semen samples were collected from 3 Phu Quoc male dogs at 15 to 24 months of age, every 5 days for each collecting time, in a total of 6 collecting times. The studied results showed that, the semen samples preserved in TCF extender + 30 mg/ml skim milk powder and TCF extender + 20 mg/ml vitamin C have given better preservation effectiveness with the average sperm motility values in the day 3rd reached 0.52 ± 0.06 and 0.65 ± 0.07 points, respectively and with the average sperm abnormality rate was 33.2 ± 0.68 and 17.9 ± 0.37%, respectively. Adding simultaneously skim milk powder (with 15mg/1ml) and vitamin C (with 20mg/1ml) in TCF extender has given better preservation effectiveness (0.70 ± 0.05 points of sperm motility values and 14.9 ± 0.63% of sperm abnormality rate in the day 3rd of preservation).