Isolation and examining several characteristics of Vibrio parahaemolyticus related to acute hepatopancreatic necrosis disease in shrimp in Tra Vinh province

Abstract

Vibrio parahaemolyticus carrying pirvp gene was considered as the causative agent of acute hepatopancreatic necrosis disease (AHPND) in shrimp. In this study, shrimp, clam and water samples from shrimp ponds and clam farms were examined for the prevalence of V. parahaemolyticus related to AHPND in shrimp. 30 shrimp samples and 20 water samples from intensive shrimp ponds and 16 white hard clam and 15 water samples from clam farms were used as materials in this study. Serotypes and antimicrobial resistance of virulent bacteria were also examined.

The studied results indicated that the pirvp gene positive V. parahaemolyticus strains were detected with 3/30 (10.0%) from shrimp samples and 1/20 (5.0%) from shrimp pond water samples. However, this pathogenic bacterium was not found from the clam and clam farm water samples. All pirvp gene positive V. parahaemolyticus strains isolated in this study belonged to O1 antigen with several K antigens. They showed high resistance to ampicillin (100%), sulfisoxazole (100%) and streptomycin (75%). This study provides some information on V. parahaemolyticus related to AHPND in shrimp in the Mekong Delta.