Study on cloning and expressing thermostable direct hemolysin (tdh) gene of Vibrio sp. bacteria isolated from red drum (Sciaenops ocellatus) raising in Thua Thien-Hue province in E. coli cell - BL21 (DE3)

Abstract

In this study, we have cloned and expressed successfully the encoded gene to create thermostable direct hemolysin (tdh) toxin antigen of Vibrio sp., which caused the ulcer disease in red drum (Sciaenops ocellatus) raising in coastal areas of Thua Thien-Hue province. The fulllength of tdh gene was 570 bp, similarity level of this gene sequence was 100% compared to the
tdh gene sequence published in the GenBank (accession number: S67841.1). The tdh genecreating an entire polypeptide sequence consisted of 189 amino acids and its similarity level was 100% compared to the polypeptide chain published in the GenBank (accession number:
iAP14018.1). The result of analyzing SDS electrophoresis showed that 6xHis-TDH fusion protein had a molecular weight of approximately 24.6 kDa