Determination and evaluation on the homologeneity of molecular genetic characteristics of CPV-2 genotypes caused acute gastroenteritis in dogs in Tien Giang province

Abstract

Canine Parvovirus, type 2 (CPV-2) is an important causative agent of the domestic and wild dogs and has been spreading in the worldwide since its appearance in the 1970s and it is one of the main pathogens causing acute gastroenteritis in dogs. Although there were a few recent studies on the circulation of CPV-2 conducted in Viet Nam, but the information on molecular
genetic characteristics of CPV-2 genotypes was very limited. To determine and evaluate the homogeneity of molecular genetic characteristics of CPV-2 genotypes circulating in dogs, VP2 gene of 40 samples of Canine Parvovirus in Tien Giang province was collected and sequenced by Sanger method on ABI 3130 system (USA) and checked the receiving sequence by BioEdit software.
The result of comparing 40 studied CPV-2 genotypes with the initial CPV-2 genotypes showed that there were 39 CPV-2 genotypes having homogeneous mutation in 4 nucleotide positions on the gene of CPV-2 at the sites (2773T, 2816C, 3790G and 3792A) determined as CPV-2c genotype. There was only one genotype of CPV-2 in this study having mutation in 3 nucleotide locations on the gene of CPV-2 at the sites (2773T, 2816C and 2817C) determined as CPV-2a genotype. The result of comparing 8 selected CPV-2c and CPV-2a genotypes in
this study with the reference CPV-2c and CPV-2a genotypes showed that there was the high similarity level on nucleotide and amino acid composition as well as the high similarity level on the molecular genetic characteristics among the studied CPV-2 genotypes in Tien Giang province; with the CPV-2 genotypes on the GenBank, with CPV-2 genotypes in vaccines on the GenBank of NCB