Some biological and immunological characteristics of porcine epidemic diarrhea virus strains isolated in the Northern Viet Nam

Abstract

This article is presented basing on the database of four porcine epidemic diarrhea virus (PEDV)
strains which were studied, isolated in the Northern Viet Nam with the aim of selecting a PEDV
strain for studying and producing vaccines against PEDV. Four field PEDV strains were identified
for their genogroup by multiplex RT-PCR technique. The studied results showed that these field
strains belonged to genogroups 2, while the attenuated vaccine strain from the imported vaccine
that currently using in Viet Nam belonged to genogroup 1. The replication of these field PEDV
strains in the vero cells were observed by immunostaining, the virus strains were infected into single cells to be majority and syncytial was small, virus titre reaching 106.1TCID50 was the highest. Immunogenicity studies between virus strains were performed by viral neutralization. As a result, PEDV 0118 field strain was 100% cross-resistant with three field strains left.