Identifying toxin types of Clostridium perfringens bacteria isolated from piggies suffering with necrotizing enteritis in six provinces in Viet Nam

Abstract

C. perfringens bacteria causes diarrhea and necrotic enteritis in the newborn pigs, this is
a reason leading to the low weight of the weaning piglets, high mortality rate, making heavy
economic loss for the pig farmers. To determine the toxin types of C. perfringens bacteria currently
circulating, causing the necrotic enteritis in the piglets in 6 provinces (Ha Noi, Thai Nguyen, Ha
Tinh, Khanh Hoa, Can Tho, Dak Lak); we isolated, assessed the biological characteristics of the
isolates and conducted genotyping by multiplex-PCR technique. As a results, there were 78 C.
perfringens bacteria strains from 124 samples isolated, accounting for 62.9%. These bacteria
strains carried full biological characteristics of the species. The results of determining genes
encoding toxin and genotyping by multiplex-PCR showed that C. perfringens bacteria strains
isolated from the piglets suffering with necrotizing enterocolitis in 6 provinces possessed type
A (94.87%) and type C (5.13%).