Cloning and expression of gene encoding Engam22 antigen of Eimeria necatrix in E. coli BL21 (DE3)

Abstract

In this study, we successfully cloned and expressed a fragment of gene encoding Engam22 antigen of Eimeria necatrix isolated from the fecal samples of chickens raised in Thua Thien - Hue province, Viet Nam. The fragment of gene encoding Engam22 antigen was cloned into plasmid pGEM®-T Easy and expressed with vector pET200/D-TOPO in Escherichia coli BL21(DE3) cells. The studied results showed
that the nucleotide sequence of fragment of gene encoding Engam22 antigen was 505 bp in length, encoding a  polypeptide chain with 167 amino acid residues, and 100% similarity to the polypeptide chain of Engam22 antigen in GenBank (accession number: AHB64327.1). The results of SDS-PAGE analysis showed that molecular weight of 6xHis-Engam22 recombinant protein was about 25 kDa.