Co-infection of circovirus and parvovirus in ducks farmed in Ha Noi, 2021

Abstract

Co-infection of duck circovirus (DuCV) and parvovirus plays an important role in beak atrophy and dwarfism syndrome (BADS) and feather shedding syndrome (FSS), causing
economic loss in duck production. This research was conducted to evaluate co-infection of the two viruses in the duck flocks and utilize a duplex polymerase chain reaction (d-PCR) method in diagnosis. A total of 48 viscera pool tissue samples were collected from clinically suspected duck flocks and diseased ducks farmed in Phu Xuyen, Ung Hoa, Thuong Tin, and Gia Lam districts of Ha Noi during April to July 2021. The studied results indicated that the positive rates for the DuCV genome according to individual sample and farm levels were 81.82% and 54.17%, respectively; for the parvovirus genome were 45.45% and 27.08%, respectively; and for co-infection of the two viral genomes were 36.36% and 18.75%. d-PCR method was first optimized and utilized to detect both the DuCV and parvovirus genomes in the samples with a high sensitivity (100%) and specificity (100%), compared to single
conventional PCR method. The finding of this study gains a better understanding of DuCV and/or parvovirus, leading to develop diagnostic methods and disease prevention strategies in duck farming.