Research on biological, molecular biology characteristics of some Canine distemper virus strains in Viet Nam in 2020

  • Trương Quang Lâm
  • Nguyễn Thị Lan
  • Đào Lê Anh
  • Nguyễn Thị Yến
  • Lê Thị Luyên
  • Nguyễn Văn Thắng
Keywords: Canine distemper virus, biological, molecular characteristics, Viet Nam

Abstract

Study on the biological and molecular biology characteristics of 3 Carreù virus strains isolated in Ha Noi City, Ninh Binh and Nghe An provinces was carried out in 2020. The culture result
showed that 3 CDV strains: VNUA.CDV.NA04, VNUA.CDV.NB05 and VNUA.CDV.HN11 multiplied rapidly on Vero-DST cells with typical lesions of syncytium with viral titers from 5.56 x 106 - 6.67 x 106 TCID50/ml. Virus began multiplying after 6 hours of infection and destroying cells (CPE) with the highest titer after 48 hours of infection. The result of sequencing and analyzing the H gene of 3 CDV strains indicated that these virus strains belonged to genotype Asia 1, located in the same phylogenetic branch of CDV strains that isolated in Viet Nam (2013-2017) and in China. This studied results will serve for researches on selecting potential virus strains to produce the bio-products for prevention and treatment of canine distemper.

điểm /   đánh giá
Published
2023-08-08
Issue
No. 4 (2022)
Section
Nghiên cứu khoa học