Determination of some virulent genes and genotype similarity of Salmonella strains in the chicken meat production chain at some districts, Ha Noi City

Abstract

The result of analysing 100 Salmonella strains isolated in the chicken meat production chain at Dong Anh, Soc Son, Gia Lam districts, Ha Noi City indicated that all of three Salmonella strains (S. typhimurium, S. enteritidis and S.albany) carried Sip B gene. Of which, 2 serotypes (100% of S. typhimurium and S. enteritidis) carried this gene and 98 % of the above mentioned Salmonella strains carried InvA gene. Of which 100% strains belonged to 2 serotypes (S. typhimurium and S. enteritidis) carried InvA.Analysis of genotypes by PFGE method from the restriction DNA of the Salmonella strains in the above mentioned chain indicated that there were 41 genotypes classified. Of which, genotype 15 including 14/250 strains accounted for the highest rate and common (5.6%), genotype 36 including 1/250 strains accounted for the lowest rate (0.4%). The genotypes were not classified including 43/250 strains (17.2%). This is base for assessment of pathological mechanism, correlation of Salmonella bacteria origin in the stages of the chicken meat production chain. Also, this is essential for disease prevention.