Determination of prevalence of Streptococcus suis type 2 in slaughtered pigs in Hue city and cloning, expression of gene encoding 6-phosphogluconate-dehydrogenase protein in E. coli BL 21

Abstract

In this study, 100 pig nasal swab samples were examined by PCR technique for detection of Streptococcus suis type 2. The studied result indicated that the prevalence of S. suis type 2 in the slaughtered pigs in Hue city was 9%. From the positive samples with S. suis type 2 through PCR technique, the bacterial strain was isolated, the gene encoding 6-phosphogluconate-dehydrogenase (6PGD) protein of this bacteria was cloned and expressed in E. coli BL21 (DE3). The length of gene encoding 6PGD protein of S. suis type 2 was 1428 bp, this gene encoding a polypeptide chain contained 475 amino acid. Similarity level of the cloned 6PGD gene in comparison with 6PGD gene of Genebank (code: gb|CP000407.1) was 99%. The result of SDS electrophoresis showed that expression of the 6PGD gene in E. coli BL21 (DE3) produced a fusion protein having molecule weigh of ~76 kDa.