Study on in vitro conservation of indigenous taro gene (Colocasia esculenta (L.) Schott).

Abstract

Currently, in vitro culture is used to produce a large number of plants that are genetically similar, uniform in growth, and disease-free in a short time. This method can preserve the precious and rare genetic resources in the long term, especially with asexual and difficult to preserve objects such as the taro plant. This study presents the results of the storage of native taro genome Cu Cang (Son La province) using in vitro culture techniques. The results showed that the optimal sterilization of root taro in the HgCl2 0.2% was 11 minutes (9 minutes for the first time and 2 minutes for the second time), reaching 62.83% of clean samples and ensure shoots grow. In vitro taro plants preserved in 1/2 MS + agar 8 g/l + mannitol 1 g/l was the most suitable, plants grow slowly, transfer time was long, and still ensure shoots growth. In vitro Cu Cang taro shoots were cultured in 1/2 MS + saccharose 90 g/l + agar 8 g/l was the most suitable for tuber production, with the highest tuber weight reaching 1.58 g.