Hypoglycemic effect of leaf extract of Pseuderanthemum crenulatum leaves in an alloxan-induced diabetic mice

Abstract

In this study, the hypoglycaemic effects of ethanol extracts from Pseuderanthemum crenulatum leaves were investigated. The evaluated trials included: in vitro α-glucosidase inhibitory activity; oral glucose tolerance test in normoglycemic mice; hypoglycemia in an alloxan- induced diabetic mice model, and oral toxicity assay. The results showed that the ethanol extract of P. crenulatum leaves was able to inhibit α-glucosidase enzyme at IC50=66.01 µg/ml, 1.8 times higher than acarbose control (IC50 was 122.2 µg/ml). After 21 days of using leaf extract of P. crenulatum (1 g/kg) to treat diabetic mice, the blood glucose was reduced by 42.02%. This result was equivalent to gliclazide (10 mg/kg) which reduced the blood glucose of diabetic mice by 41.78%. The oral toxicity test results at a dose of 5 g/kg equivalent to 357 g of fresh leaf/kg after 72 hours was not exhibited acute oral toxicity in mice. In summary, there is a need for more studies on the ability of P. crenulatum leaves to treat diabetes.