Development and validation of quantitative procedure of clotrimazole and eugenol in gel product using high-performance liquid chromatography

Abstract

Vulvovaginitis is a prevalent gynaecological ailment worldwide, often attributed to bacteria, Candida fungi, or Trichomonas. The synergistic action of antifungal agents and essential oils can enhance the efficacy against pathogenic microorganisms. In this study, a simultaneous quantitative method for determining clotrimazole and eugenol in a laboratory-compounded gel product was developed using high-performance liquid chromatography
with a photodiode array detector (HPLC-PDA)...