STUDY ON CREATION OF IN VITRO ROOTS AND SUBSTRATES IN THE ARBORETUM OF Orthosiphon aristatus PLANTLETS

Abstract

Orthosiphon aristatus is well- known for a popular medical plant with the uses to treat kidney stones, hypoglycemia, acute and chronic nephritis, gout, rheumatism, hepatitis , diabetes mellitus, cancer … This article presents the results of effects of auxin, substrates on root formation, growth and development of Orthosiphon aristatus from Laos in order to contribute to finding a in vitro multiplication process to develop this plant in Vietnam. The optimum medium fomula for the root formation of Orthosiphon aristatus is the basal MS medium supplemented with a- NAA 0.8 mg/l, a number of roots per a shoot are 10,80, the medium length of a root is 5,21cm after 8 culturing weeks. The suitable medium for them to develope at the nursery stage before growing in the mass- production is medium soil: sand: burned husk with the ratio of 2: 1: 1.