IDENTIFICATION OF THREE SPECIES, Paris fargesii, Paris polyphylla, Paris vietnamensis COLLECTED IN VIET NAM AND PHYLOGENETIC INFERENCE IN THE GENUS Paris

Abstract

Species of the genus Paris are precious medicinal herbs, which consist of many kinds of pharmaceutical substances with homeostasis effect, antibacterial, antiviral, anti-inflammatory and detoxification activities as well as inhibition of cancer cells. However, due to overexploitation of these species to produce pharmaceuticals, the number of individuals in each species of the Paris genus declined dramatically, which is why it is necessary to carry out emergency conservation measures for the Paris species. Therefore, the collection, identification and phylogenetic analysis of Paris species are a basis for conduction of conservation and development measures for species of the genus Paris in Vietnam. In this study, three species of the genus Paris (Paris fargesii, Paris polyphylla var chinensis, Paris vietnamensis) collected in Lai Chau, Lang Son and Thanh Hoa (Vietnam) were identified based on comparative morphology and DNA barcode including ITS and matK. Phylogenetic tree was established based on nucleotide sequences of ITS region and matK gene isolated from three Paris species of P. fargesii, P. polyphylla var chinensis, P. vietnamensis in Vietnam and ITS and matK sequences of 21 Paris species on GenBank using Maximum Likelihood method has shown initial results of evolution of Paris species.