ISOLATION AND SELECTION OF BACILLUS STRAINS WITH CAPABILITY IN PRODUCTION OF POLYGLUTAMIC ACID

Abstract

Curently, attentions are drawn to studies about application of active substances produced by microbes in environmental treatment. Polyglutamic acid (PGA) is one of biological polymers that has multiple applications in different fields and has a great potential use as biological coagulant in water treatment. Bacteria under Bacillus group are useful in production of biologically active substances and have a significant role in PGA production. This study aimed to isolate and select Bacillus bacteria from fermented soya been product in order to provide seed strain for further investigations on production and application of PGA in the role of biological coagulant. After using isolation, selection and verification methods by FT-IR infrared spectroscopy, four Bacillus strains from Natto fermented soybean products were obtained, of which two strains have been selected with better PGA production performance B11, B33, is a prerequisite for PGA production for further studies.