SURVEY QUICK DNA EXTRACTION PROTOCOLS - PROPOSITION OF A DNA EXTRACTION PROCESSES THAT IS OPTIMAL FOR DNA PATERNITY TESTING

Abstract

After experiment, the process used NaOH - SDS combinate with thermal shock was found to be the DNA extraction method more efficient than the other. Comparing extraction efficiency at different SDS concentrations to propose the optimal extraction procedure, the result showed that 0.01% is the suitable SDS concentration. To evaluate the effectiveness of the improved extracting procedure, we compared it with a commercially available kit (E.Z.N.A Forensic DNA kit) on 10 samples and applicated to extract DNA on 200 samples. After extracted, all samples have been used for STR-PCR reaction. On 10 samples, average fluorescence signal from the improved extraction procedure is lower than the commercial kit, but the results were accurate and consistent. On 200 samples, the results showed that no samples were inhibited, more than 90% of samples for PCR product had fluorescence intensity above 100 RFU and 100% for signal above 50 RFU. On the samples have been known the parentage relationship, the result is match.