STUDY ON IN VITRO PROPAGATION OF Dendrobium nobile Lindl.

Abstract

Dendrobium nobile Lindl. gives beautiful flowers and has medicinal value. Currently, this natural orchid plant in any forest is overexploited, leading to depletion, therefore, it should be preserved. In this study, the effects of factors on protocorm regeneration,  shoot growth and development, rooting, and the survival and growth of seedlings were investigated. Results showed that Murashige and Skoog medium supplemented including sucrose 10 gL^-1; activated charcoal 1 gL^-1; BAP 1 mgL^-1 and coconut water 100 mL^-1 were the best for protocorm regeneration. A suitable medium for the growth and development of orchid shoots Dendrobium nobile Lindl. in vitro was Murashige and Skoog medium supplemented including saccharose 25 gL^-1; activated charcoal 1 gL^-1; BAP 1 mgL^-1 and potato 50 gL^-1. Additional volume of 0.5 mgL^-1 NAA to the culture medium promoted the best root formation (root length up to 20.5 mm, number of roots/shoot reaching 7.32, long strong roots). Seedlings were grown in a mesh house on a mixture of decomposed sawdust and crumbs at the ratio of 1: 1 which provided the best results after 60 days (rate of living plants reaching 92%; strong trees with many roots, the number of newly formed shoots reaching 2.86 buds/tree).