MORPHOLOGICAL, CULTURAL CHARACTERISTIC AND NUTRITIONAL CONTENT OF Ganoderma lucidum Lim xanh COLLECTED IN LANG SON PROVINCE

Abstract

The research was carried out to collect and conserve genetic resource of Ganoderma lucidum from nature. Fruiting bodies of Ganoderma lucidum were discovered at natural forest in Huu Lan commune, Loc Binh district, Lang Son province. Analysis of ITS 1 - 5.8S rRNA - ITS 2 gene sequence showed that strain Lim xanh is a member of the genus Ganoderma and named as Ganoderma lucidum Lim xanh. Research results revealed that using 70% ethanol for 7 minutes gives the highest efficiency in isolating pure mycelium from the fruiting, with 26.67% of the samples being successful. Strain Lim xanh was ability to grow on four different media, including PDA, MCM, YMA, PL; in which the PDA medium is the most optimum for growth of  strain Lim xanh. Fruiting bodies of Ganoderma lucidum Lim xanh cultivated on artificial condition were fan-shaped, dark brown, diameter of fruit bodies reaching of 5-8 cm, stalk length 7-10 cm and 5.43 - 10.64g of fruit weight. Analysis result of nutritional contents of Ganoderma lucidum contained 15.46% total protein, carbohydrates (41.42%), fiber (37.70%) and minerals (2.45%).