STUDY ON RADIOPROTECTION EFFECT FOR HUMAN LYMPHOCYTES AND FIBROBLASTS OF RESVERATROL-ENCAPSULATED LIPOSOME

Abstract

This study aims to evaluate radioprotection effect of resveratrol-encapsulated liposomes (Lip-RES). Firstly, Lip-RES was prepared by a combining method of lipid-film hydration method and sonication. Penetration of Lip-RES into human lymphocytes and human fibroblasts (HF cells) was observed by fluorescene microscopy. The results demonstrated that Lip-RES was successfully prepared with a relatively spherical shape, good characteristics (mean particle size of 103.9 nm, polydispersity index of 0.211, zeta potential of -31.9 mV, encapsulation efficiency of 92.31% and payload of 8.47%), and a relatively high stability. Lip-RES was well penetrated into human lymphocytes and HF cells. Under non-irradiated condition, Lip-RES showed a negligible genotoxicity, but exhibited a significant cytotoxicity for lymphocytes and HF cells at RES concentration of more than 10 µg/mL. Under irradiated condition at a dose of 2 Gy, compared to the control, Lip-RES could decrease the percentage of cell death of 16-30% and decrease the number of foci/cell of 10-15%. This indicates the radioprotection effect of Lip-RES for human lymphocytes and fibroblasts. Moreover, at a RES concentration of 2.5 µg/mL, Lip-RES showed a best radioprotection.