MORPHOLOGICAL AND CULTURAL CHARACTERISTICS OF PHELLINUS SPECIES COLLECTED IN THE NORTH MOUNTAIN PROVINCES, VIET NAM

Abstract

This study was performed to collect genetic resources of Phellinus species in nature reserves in the North of Vietnam and determine optimal method to process the samples during isolation of mycelium, as well as finding optimal media for cultivation of the mycelium. In this study, four fruiting bodies of Phellinus species were collected, name designated as NTH-PL1, NTH-PL2, NTH-PL3 and NTH-PL4. The fruiting bodies of Phellinus species has a semicircle/horseshoe shape, dark brown color, diameter of the body ranging from 5.0 to 15.3 cm. Isolation results show that using 70% ethanol for 10 minutes was the most suitable to remove microbial contamination in the samples, with an efficiency of 29.17%. Four strains of Phellinus species were able to growth in PDB, MCM, MEB and YEB medium, in which MCM medium was the most suitable for strain NTH-PL1 (10.6 mg of dry filamentous biomass in 100 ml of medium), NTH-PL2 (10.4 mg/100 ml), NTH-PL3 (13.9 mg/100 ml) and NTH-PL4 (16.4 mg/100 ml).