OPTIMIZATION OF AN ULTRASOUND-ASSISTED EXTRACTION CONDITION FOR ALKALOIDS COMPOUNDS FROM RHIZOMES OF Curcuma zedoaria USING RESPONSE SURFACE METHODOLOGY

Abstract

Response surface methodology using a Box-Behnken design was employed to optimize the conditions for extraction of alkaloids from the rhizomes of Curcuma zedoaria. Alkaloids from the rhizomes of Curcuma zedoariaare subjected to ultrasonic-assisted extraction. Four independent variables such as: ultrasonic temperature, ethanol concentration, material/ solvent ratio, and ultrasonic time were investigated. In which, ultrasonic temperature, ethanol concentration, and ultrasonic time have a lot of influence on alkaloids contentand and are used to optimize for rhizomes of Curcuma zedoaria. The results showed that the experimental data could be fitted to a quadratic polynomial model using correlation analysis of the mathematical regression model. Response surface plots showed that all independent variables significantly affected the alkakoid content extracted from rhizomes of Curcuma zedoaria. The optimum extraction conditions were as follows: ethanol concentration of 69% (v/v), ultrasonic temperature of 62^oC, ultrasonic time of 15 min, and the ratio of material  to solvent of 1:30 (w/v) . The alkaloids content yield under the optimum conditions was found to be 289.25±0.43 mg AE/g extract, which agreed with the predicted value of 288.24 mgAE/g extract.