USING DNA BARCODES TO IDENTIFY THAI NGUYEN CATFISH

Abstract

The mitochondrial cytochrome c oxidase subunite I (COI) gene has been used extensively to study molecular taxonomy in animals. In this study, we study the sequencing analysis of COI gene segments isolated from Thai Nguyen catfish sample. COI gene fragment was isolated from catfish genome DNA by PCR technique. Then, the PCR product was purified and sequenced, the COI gene segment obtained was 616 bp in length. Nucleotide sequences of COI gene were compared with those published on Genbank using Blast software. The comparison results showed that the studied COI gene segment did nothave scientific basis to identify species in the genus Silurus. Using Bioedit software to compare the nucleotide sequence of the COI gene fragment showed that the studied catfish sample could be a different species compared with the submitted species belonging genus Silurus.