STUDY ON IN VITRO PROPAGATION OF Dendrobium asomum Lindl. DISTRIBUTE IN THAI NGUYEN PROVINCE

Abstract

This study aims to investigate influential factors to species Dendrobium asomum Lind. in vitro propagation such as protocorm regeneration,  shoot growth, development, rooting, the survival and growth of seedlings were investigated. Results showed that Murashige and Skoog medium supplemented including 10 gL^-1 sucrose; 1 gL^-1activated charcoal; 0,2 mgL^-1 NAA; 2 mgL^-1 BAP were the best for protocorm regeneration. A suitable medium for the growth and development of Dendrobium amosum Lindl. shoots in vitro was Murashige and Skoog medium supplemented including 25 gL^-1 sucrose; 1 gL^-1activated charcoal; 1 mgL^-1 BAP and 50 gL^-1 potato. Additional volume of 0.5 mgL^-1 NAA to the culture medium promoted the best root formation (root length up to 5.1 mm, number of roots/shoot reaching 3.0 after 80 days, long strong roots). Seedlings grown in a mesh house on the condition of forest moss was suitable, supporting the first stage plants with ½ MS solution for good results (rate of living plants reaching 95%; strong trees with many roots, newly shoots grows well).