CHARACTERISTICS OF NUCELOTIDE SEQUENCE OF SSR MARKER IN GENE CODED SUCROSE SYNTHASE FROM GREEN AND PURPLE TRUNG DU TEA VARIETIES

Abstract

cDNA/EST - derived SSR markers (single sequence repeat-SSR) has the advantage of reducing the cost and time of directing the marker because cDNA/EST fragments are directly related to functional genes. In this study, we conducted an analysis of the nucleotide sequence of SSR marker of the gene encoding sucrose synthase - the enzyme involved in sucrose synthesis as a basis for molecular markers applied in tea breeding. Motif TTGTT belongs to 3’ UTR (untranslated region) of the gene encoding sucrose synthase was repeated in two samples of the interrupted group by a few bases that are not motif structure, the motif sequence obtained in green and purple Trung du tea, respectively: (TTGTT) 4 GTT (TTGTT) 4; (TTGTT) 6 GTT (TTGTT) 3; (TTGTT) 5 GTT (TTGTT). Comparing the TTGTT sequence of the sucrose synthase encoding gene with the published sequences shows that the TTGTT mottif has a conservative in varieties and a high polymorphism among the analyzed tea varieties.