Study on the development of an extraction process and evaluation of certain properties of the extract from perilla (Perilla frutescens var. crispa) leaves

Abstract

Perilla (Perilla frutescens var. crispa) leaves are widely used in traditional medicine and nutrition, and are known for their richness in polyphenols and bioactive compounds. This study aimed to optimize the extraction and enrichment of polyphenols from perilla leaves collected in Dong Anh commune, Hanoi city. The optimal extraction conditions were determined as 70° ethanol, a solvent-to-material ratio of 10:1 (v/w), at 35 °C for 65 minutes, yielding 5.74 mg GAE/g dry material. The crude extract contained a total polyphenol content of 114.83 mg GAE/g, which increased to 135.16 mg GAE/g after enrichment using activated X5 resin. In addition, the enriched extract gained a rosmarinic acid content of 62.8 mg/g. These findings demonstrate that the optimized extraction process combined with X5 resin enrichment produces a polyphenol-rich extract from perilla leaves, with promising applications in pharmaceuticals and functional foods.