DNA DIVERSITY OF BACTERIAL STRAINS (PSEUDOMONAS SOLANACEARUM) CAUSED BACTERIAL WILT DISEASE IN PEANUT BY RAPD MARKERS

Abstract

Bacterial wilt disease of peanut caused by pseudomonas solanacearum is among the majordiseases causing significant yield loss in peanut. Molecular marker analysis of bacterial strains would be of use for peanut breeders, geneticists and taxonomists. Twenty RAPD primers werescreened for their ability to reveal polymorphism between sixty colected pesudomonassolanacearum strais in the different local of bacterial wilt disease. Overall, 20 markers employedin this study revealed polymorphism, with a total of 616 fragments observed in the 6 baterial strains screened with values PIC from 0 to 0,48.The dendrogram was divided into two the main group. The first group includes a strain P4at a dissimilarity threshold of 28% in the dendrogram. The second group includes 5 strais,among two strains P3 and P5 have lowest genetic coefficient 98%.