Research on microcapsules for IgY antibodies

Abstract

IgY antibodies have been shown to be effective in the prevention and treatment of some infectious diseases, such as influenza A and hand, foot and mouth disease (HFMD)... Chitosan-alginate microcapsules are considered an effective approach to delivering IgY into the body via the oral route. The research on IgY microcapsules for IgY antibodies investigated the effects of encapsulating agents and encapsulation conditions on the efficiency of the microcapsules
process of IgY antibodies against Enterovirus 71 (EV71), including: alginate concentration, chitosan concentration, microcapsules environment pH. The results determined the optimal parameters to enhance the efficiency of microcapsules IgY, which are 2.5% (w/v) sodium alginate, 0.2% chitosan, and microcapsules environment pH 3,5. Under optimum conditions, IgY microcapsules efficiency was 63.93%. After freeze-dried microcapsules, the activity against EV71 in the microcapsules was 35.01%. The IgY stability in simulated gastric fluid (SGF) was significantly improved when encapsulated by chitosan-alginate microcapsules, and the activity of IgY was fully maintained for 3 hours in SGF with the appearance of pepsin. Scanning electron microscopy (SEM), results showed that the
microcapsule particle size is ≤10 μm, the microcapsule particle structure is porous, and there are many microcapsule particles in the microcapsule product after freeze-drying.